人肝癌细胞SK-HEP-1

简要描述：青旗（上海）生物技术发展有限公司所提供的人肝癌细胞SK-HEP-1质量可靠、规格齐全,青旗（上海）生物技术发展有限公司不仅具有专业的技术水平,更有良好的售后服务和优质的解决方案,欢迎您来电咨询此产品具体参数及价格等详细信息！

更新时间：2022-12-11

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详情介绍

品牌	自营品牌	货号	BFN60800688
规格	T25培养瓶x1 1.5ml冻存管x2	供货周期	现货
主要用途	仅供科研	应用领域	医疗卫生,生物产业

细胞名称	人肝癌细胞SK-HEP-1
货物编码	BFN60800688
产品规格	T25培养瓶x1	1.5ml冻存管x2
细胞数量	1x10^6	1x10^6
保存温度	37℃	-198℃
运输方式	常温保温运输	干冰运输
安全等级	1
用途限制	仅供科研用途

培养体系	DMEM高糖培养基（Hyclone）+10%胎牛血清（Gibco）+1%双抗（Hyclone）
培养温度	37℃	二氧化碳浓度	5%
简介	人肝癌细胞SK-HEP-1细胞已被鉴定为内皮来源。该细胞系为异倍体女性人（XX），染色体在亚三倍体范围内。在裸鼠中，它能形成与肝癌相一致的大细胞癌。人肝癌细胞SK-HEP-1细胞由青旗（上海）生物技术发展有限公司于2017年引种自ATCC(HTB-52)。
注释	Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Part of: MD Anderson Cell Lines Project. From: Memorial Sloan Kettering Cancer Center; New York; USA. Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-535. Characteristics: Has lost chromosome Y. Doubling time: 46.7 +- 10.3 hours, 94.2 hours (in CDM4-CHO medium), 289.8 hours (in 293 SFM II medium) (PubMed=25822314); ~30 hours (DSMZ). Microsatellite instability: Stable (MSS) (Sanger). Omics: Deep exome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: Protein expression by reverse-phase protein arrays. Omics: Secretome proteome analysis. Omics: SNP array analysis. Omics: Transcriptome analysis.
STR信息	Amelogenin：X,X；CSF1PO：11,12；D12S391：18,18；D13S317：8,12；D16S539：12,12；D18S51：13,15；D19S433：12,15.2；D21S11：29,31；D2S1338：20,23；D3S1358：16,16；D5S818：10,13；D6S1043：11,11；D7S820：8,11；D8S1179：13,14；FGA：17,17；PentaE：13,21；TH01：7,9；TPOX：9,9；vWA：14,17；
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验收细胞注意事项

1、收到人肝癌细胞SK-HEP-1细胞，请查看瓶子是否有破裂，培养基是否漏出，是否浑浊，如有请尽快联系。

2、收到人肝癌细胞SK-HEP-1细胞，如包装完好，请在显微镜下观察细胞。,由于运输过程中的问题，细胞培养瓶中的贴壁细胞有可能从瓶壁中脱落下来，显微镜下观察会出现细胞悬浮的情况，出现此状态时，请不要打开细胞培养瓶，应立即将培养瓶置于细胞培养箱里静止 3-5 小时左右，让细胞先稳定下，再于显微镜下观察，此时多数细胞会重新贴附于瓶壁。如细胞仍不能贴壁，请用台盼蓝染色法鉴定细胞活力，如台盼蓝染色证实细胞活力正常请按悬浮细胞的方法处理。

3、收到人肝癌细胞SK-HEP-1细胞后，请镜下观察细胞，用恰当方式处理细胞。若悬浮的细胞较多，请离心收集细胞，接种到一个新的培养瓶中。弃掉原液，使用新鲜配制的培养基，使用进口胎牛血清。刚接到细胞，若细胞不多时血清浓度可以加到 15％去培养。若细胞迏到 80%左右，血清浓度还是在 10％。

4、收到人肝癌细胞SK-HEP-1细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养基吸出，留下 5-10ML 培养基继续培养：超过 80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液，1000 转/分钟离心 3 分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

5、将培养瓶置于 37℃培养箱中培养，盖子微微拧松。吸出的培养基可以保存在灭菌过的瓶子里，存放于 4℃冰箱，以备不时之需。

6、24 小时后，人肝癌细胞SK-HEP-1细胞形态已恢复并贴满瓶壁，即可传代。（贴壁细胞）将培养瓶里的培养基倒去，加 3-5ml（以能覆盖细胞生长面为准）PBS 或 Hanks’液洗涤后弃去。加 0.5-1ml 0.25%含 EDTA 的胰酶消化，消化时间以具体细胞为准，一般 1-3 分钟，不超过 5 分钟。可以放入37℃培养箱消化。轻轻晃动瓶壁，见细胞脱落下来，加入 3-5ml 培养基终止消化。用移液管轻轻吹打瓶壁上的细胞，使之*脱落，然后将溶液吸入离心管内离心，1000rpm/5min。弃上清，视细胞数量决定分瓶数，一般一传二，如细胞量多可一传三，有些细胞不易传得过稀，有些生长较快的细胞则可以多传几瓶，以具体细胞和经验为准。（悬浮细胞）用移液管轻轻吹打瓶壁，直接将溶液吸入离心管离心即可。

7、贴壁细胞，悬浮细胞。严格无菌操作。换液时，换新的细胞培养瓶和换新鲜的培养液，37℃，5%CO2 培养。

特别提醒： 原瓶中培养基不宜继续使用，请更换新鲜培养基培养。

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